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Author
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Hyuna Lee
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Biological Sciences
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Hyuna
Lee, working under Professor Jeon, used a unique microfluidic
platform to perform her biological research. Using this
platform, she was able to culture neurons to isolate the
axons from the soma, so that she could observe GFP-tagged
mitochondrial movement in the axons. Through her research
experience, Hyuna developed an appreciation for reading
papers describing how other researchers conducted their
studies. She found the constant friendship and support
of the people in her lab to be particularly valuable. Hyuna
intends to attend graduate school to further develop the
passion she has developed for innocent lives shortened
by incurable diseases.
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Abstract
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Neurodegenerative
diseases, such as Alzheimer’s
disease, lead to permanent disabilities in muscular movements,
memory and dementia. Mitochondrial transport is closely linked
to neuronal function. Mitochondrial trafficking along microtubules
delivers energy in the form of adenosine triphosphate (ATP),
which is necessary for signal transmission and sustaining viability.
Controversy remains regarding the direction and mechanisms
by which mitochondria move in aging and diseased neurons. It
is unclear whether axonal transport impairment is due to mutations
in mitochondrial DNA or other cytoplasmic factors. We used
a microfluidic culture platform to analyze mitochondrial trafficking
patterns in axons by transfecting neurons with Mito-GFP and
observing them under time-lapse microscopy to analyze differences
in mitochondrial morphology, movement and interaction. Elongated
mitochondria in soma and dendrites were less motile but moved
steadily. Mitochondria in axons generally had short ovular
shapes with rapid bidirectional movements and varying speeds.
Many clusters of mitochondria were found in locations that
required large amounts of ATP. We also observed mitofusion
or mitofission, which may be indicators of neuron survival
or death, respectively. This research is a first step in developing
a model to closely reflect neuronal mitochondrial trafficking
patterns.
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Faculty
Mentor
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Understanding
the relationship between mitochondrial axonal transport
and neurodegenerative diseases such as Alzheimer’s,
Huntington’s and Parkinson’s is important in the
search for successful treatments for these diseases. In tracking
mitochondrial movement, it is important to distinguish the
morphology of the dendrites and axons. The microfluidic culture
platform Hyuna Lee used in her research establishes this distinction,
providing a clear look at trafficking patterns throughout the
neuron. The results of this study offer a better understanding
of mitochondrial transport, a potential first step toward complete
elucidation of the pathology of neurodegenerative diseases.
Hyuna’s work demonstrates the success that can be achieved
by undergraduate students who passionately devote themselves
to their research.
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If
you wish to view the paper in its entirety, please select
the link given to the PDF file.
[01_lee.pdf]
If you wish to download the Adobe Acrobat Reader,
please go to Adobes website (www.adobe.com).
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© 2007
by the Regents of the University of California. All rights reserved.
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